PCI Neuroscience

180

Title *

The Switchmaze: an open-design device for measuring motivation and drive switching in miceuse asterix (*) to get italics

Authors *

Clara Hartmann, Ambika Mahajan, Vinicius Borges, Lotte Razenberg, Yves Thönnes, Mahesh M. KarnaniPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

Year *

2024

Picture *

Abstract *

<p>Animals need to switch between motivated behaviours, like drinking, feeding or social interaction, to meet environmental availability, internal needs and more complex ethological needs such as hiding future actions from competitors. Inflexible, repetitive behaviours are a hallmark of many neuropsychiatric disorders. However, how the brain orchestrates switching between the neural mechanisms controlling motivated behaviours, or drives, is unknown. This is partly due to a lack of appropriate measurement systems. We designed an automated extended home-cage, the Switchmaze, using open-source hardware and software. In this study, we use it to establish a behavioural assay of motivational switching in mice. Individual animals access the Switchmaze from the home-cage and choose between entering one of two chambers containing different goal objects or returning to the home-cage. Motivational switching is measured as a ratio of switching between chambers and continuous exploitation of one chamber. Behavioural transition analysis is used to further dissect altered motivational switching. As proof-of-concept, we show environmental manipulation, and targeted brain manipulation experiments which altered motivational switching without effect on traditional behavioural parameters. Chemogenetic inhibition of the prefrontal-hypothalamic axis increased the rate of motivation switching, highlighting the involvement of this pathway in drive switching. This work demonstrates the utility of open-design in understanding animal behaviour and its neural correlates.</p>

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https://doi.org/10.17605/OSF.IO/YB67QYou should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://

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Indicate the full web address (DOI, SWHID or URL) giving public access to these codes (if you have any problems with the deposit of your codes, please contact contact@neuro.peercommunityin.org). In case all raw codes are included in the preprint, indicate the DOI or URL of the preprint. *

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Keywords (optional)

motivation, switching, feeding, mouse, automated behavioral recording

Methods that require specific expertise (optional)

NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).

Thematic fields *

Behavioral/Cognitive Neuroscience, Methods development, Rodent model system

Suggested reviewers - Suggest up to 10 reviewers (provide names and Email addresses). (Optional)

Eric Burguire eric.burguiere@icm-institute.org, Andre Chagas A.Maia-Chagas@sussex.ac.uk, Tim Murphy thmurphy@mail.ubc.ca, Andrew MacAskill ucgbafm@gmail.com, Jonny Saunders j@nny.fyi, Thomas Akam thomas.akam@psy.ox.ac.uk, Julija Krupic jk727@cam.ac.uk, Ewelina Knapska e.knapska@nencki.gov.pl, Balasz Hangya hangya.balazs@koki.hu, David Wolfer davidp.wolfer@uzh.ch, Alexxai Kravitz alexxai@email.wustl.edu, Andreas Schaefer andreas.schaefer@crick.ac.uk, David Redish redish@umn.edu, Julia Harris julia.harris@crick.ac.uk, Ede Rancz ede.rancz@inserm.fr, Vitor Lopes dos Santos [vtlsantos@gmail.com] suggested: Andressa Radiske (andressa.radiske@isd.org.br), Vitor Lopes dos Santos [vtlsantos@gmail.com] suggested: Stephen McHugh (stephen.mchugh@pharm.ox.ac.uk)

e.g. John Doe john@doe.com

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Submission date

2024-02-13 18:58:48

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Balázs Hangya

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