GULYAS Attila I.
- Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
- Electrophysiology, Interneurons, Systems/Circuit Neuroscience, Neuronal oscillations
Areas of expertise
MSc in Biology, Eötvös Loránd University, Budapest, Hungary Ph.D. on anatomy of hippocampal interneurons, Hungarian Academy of Sciences, Budapest, Hungary DSc.: Hungarian Academy of Sciences, Budapest, Hungary Major field of training and research experience: -Neuroanatomy 1989-2005: light and electron microscopy, immunostaining, track tracing, IEM-HAS Subcortical innervation of the hippocampus. Classification of hippocampal inhibitory neuron types. Studying the role of different types of interneurons in the regulation of hippocampal activity. Estimation of the total number, ratio and distribution of excitatory and inhibitory inputs on different subsets of hippocampal interneurons and pyramidal cells. Study of the anatomical basis of the interaction between the hippocampus and the medial septum. -Electrophysiology 1994-95: in vitro electrophysiology of hippocamal inhibitory neurons combined with anatomy, Pasteur Institute, Richard Miles Interactions between excitatory and inhibitory neurons in the CA3 area of the hippocampus in vitro. Combined electrophysiological, pharmacological and anatomical studies of inhibitory cells in the in vitro hippocampal slice. 2006-present: in vitro electrophysiology of hippocampal network dynamics, IEM-HAS Characterization of different types of interneurons in the hippocampal formation, on the basis of their connectivity, neurochemical marker content and electrophysiological properties. How do interneurons integrate convergent synaptic inputs and generate action potentials during different network activity patterns. How do the different cell types balance the activity of excitatory neurons as a function of network activation and synchrony? How do they contribute to the generation to different network patterns, characterized with different dynamics (in vitro). We use an improved in vitro slice preparation that upon pharmacological manipulations can switch among different activity patterns similar to physiological (gamma oscillation, sharp-wave ripples) and pathological (epileptic) in vivo patterns. This arrangement allows selective and quick manipulation of the network's behavior and parallel multichannel recording of network activity (field potentials and multiunit) as well as recording of cellular output (loose-patch recording of action ptentials) and input (voltage and current clamp) from identified neurons or pairs of neurons.